中文字幕在线观看|中文字幕在线播放|最新中文字幕在线|中文字幕第一页在线|最新中文字幕在线观看|在线播放中文字幕|日本中文字幕在线|中文字幕视频在线观看|中文字幕在线播放|日本电影中文字幕在线观看|伊人免费在线观看高清版|日韩中文字幕影视一区|国产中文字幕影视二区

The expression dysregulation of microRNAs (miRNA) has been widely reported during cancer development, however, the underling mechanism remains largely unanswered. In the present work, we performed a systematic integrative study for genome-wide DNA methylation, copy number variation and miRNA expression data to identify mechanisms underlying miRNA dysregulation in lower grade glioma. We identify 719 miRNAs whose expression was associated with alterations of copy number variation or promoter methylation. Integrative multi-omics analysis revealed four subtypes with differing prognoses. These glioma subtypes exhibited distinct immune-related characteristics as well as clinical and genetic features. By construction of a miRNA regulatory network, we identified candidate miRNAs associated with immune evasion and response to immunotherapy. Finally, eight prognosis related miRNAs were validated to promote cell migration, invasion and proliferation through in vitro experiments. Our study reveals the crosstalk among DNA methylation, copy number variation and miRNA expression for immune regulation in glioma, and could have important implications for patient stratification and development of biomarkers for immunotherapy approaches. A miRNA multi-omics study reveals not only mechanisms for miRNA dysregulation, but also the tumor heterogeneity and immunological diversity within low grade glioma, and presents better prognostic value than traditional molecular markers.