| Comprehensive investigation in oncogenic functions and immunological roles of NCBP2 and its validation in prostate cancer | |
| 論文作者 | Wang, J; Guo, T; Zhang, XM; Guo, JC; Meng, XY; Yan, S; Wang, Y; Xiao, YT; Xu, WD; Wei, XD; Ding, KK; Zhang, J; Mi, YY; Wu, S; Chen, J; Huang, YH; Ren, SC; Hou, JQ |
| 期刊/會(huì)議名稱 | TRANSLATIONAL ONCOLOGY |
| 論文年度 | 2024 |
| 論文類別 | |
| 摘要 | Background: Nuclear cap-binding protein 2 (NCBP2), as the component of the cap-binding complex, participates in a number of biological processes, including pre-mRNA splicing, transcript export, translation regulation and other gene expression steps. However, the role of NCBP2 on the tumor cells and immune microenvironment remains unclear. To systematically analyze and validate functions of NCBP2, we performed a pan-cancer analysis using multiple approaches. Methods: The data in this study were derived from sequencing, mutation, and methylation data in the TCGA cohort, normal sample sequencing data in the GTEx project, and cell line expression profile data in the CCLE database. Results: Survival analyses including the Cox proportional-hazards model and log-rank test revealed the poor prognostic role of NCBP2 in multiple tumors. We further validated the oncogenic ability of NCBP2 in prostate cancer cell lines, organoids and tumor-bearing mice. A negative correlation was observed between NCBP2 expression and immune score by the ESTIMATE algorithm. Simultaneously, the NCBP2-induced immunosuppressive microenvironment might be related to the decline in CD8+ T cells and the increase in regulatory T cells and neutrophils, examined by flow cytometry experiments for NCBP2 overexpressed tumor-bearing mice. Conclusion: This research offered strong proof supporting NCBP2 as the prognostic marker and the therapeutic target in the future. |
| 卷 | 47 |
| 影響因子 | 4.5 |
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